Tilt Light Sheet Imaging System

The Tilt is a modular light sheet imaging system that can be easily added to virtually any inverted microscope. It can work with both low and high magnification objectives, even high NA oil objectives. With incredibly low levels of photobleaching and phototoxicity, the Tilt is ideally suited for long term imaging experiments of living organisms to single cells.

Features Specifications Applications How the Tilt works Multimodal imaging Movies Downloads

Features

Tilt Features

Minimal photobleaching and phototoxicity
Compatible with a wide range of sample types
Adapts to most inverted microscopes
Does not interfere with transmitted light and other modes of illumination
Designed to work with both high and low NA objectives
No image reconstruction needed
Simple to use
Accepts any single mode laser
Compatible with any camera
Compatible with most imaging software packages

Optional temperature control

Temperature control via custom chamber from OkoLabs. Heating, cooling and gas regulation all available.

Specifications

Applications

How the Tilt works

schematic

The Tilt brings Lateral Interference Tilted Excitation (LITE) microscopy (Fadero, et al., 2018) to the market. LITE microscopy eliminates the steric interference between illumination and detection objectives found in conventional light sheet illumination methods, thus making it compatible with any detection objective, including high NA objectives (1.4 and greater). The Tilt system includes the LITE illumination module and an X,Y, PiezoZ substage, enabling high-speed, high-resolution, 3D light sheet imaging on a standard inverted microscope.

Multimodal imaging

The Tilt can be added to most any inverted microscope w/o impacting the existing modalities on that microscope. As such, the Tilt is very well suited to add a second imaging modality to an existing microscope system. These includes adding the Tilt to a spinning disk confocal, TIRF, SIM or point scanning confocal microscope. In addition, the Tilt light sheet imaging system allows for DIC and other contrast enhancing brightfield techniques to be done alone side light sheet imaging.

Movies

Downloads

expanded MT and Nuclear001 - Deconvolved, TypeAutomatic_crop_crop_crop - Movie

expanded ER MITO and Nuclear003 - Deconvolved, TypeAutomatic_crop_crop - Movie